Axel Walch and Mirko Peitzsch
Dr. rer. nat. Mirko Peitzsch
Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universität Dresden
Prof. Dr. Axel Walch
Research Unit Analytical Pathology, Helmholtz Zentrum München
Christian Greunke – Postdoc
Na Sun – Postdoc
Paal Wallace – Postdoc
Project S01 provides centralized highly sophisticated analytical tools, which are crucial in the context of adrenal research due to the need of accurate measurements of adrenal hormones, such as catecholamine metabolites and steroids. Besides targeted quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) based methodologies, further screening platforms using high-resolution mass spectrometry, such as MALDI-mass spectrometry imaging in the context of tissue-based research, for biomarker discovery etc., are available. All those mass spectrometry based methodologies provide a powerful tool to uncover changes in metabolism associated with adrenal diseases.
Analytical platform – Specific Aims
(I) Identifcation of potential biomarkers and investigation of tumour heterogeneity by MALDI-Mass Spectrometry Imaging. Validation of potential clinically relevant biomarkers in liquid biopsies by LC-MS/MS.
(II) Improvement and development of new methodologies for quantitative LC-MS/MS based analysis.
(III) Transfer technologies to other institutions and translation of precise and accurate research based analytical tools into routine clinical applications.
N. Sun, Y. Wu, K. Nanba, S. Sbiera, S. Kircher, T. Kunzke, M. Aichler, S. Berezowska, J. Reibetanz, W. E. Rainey, M. Fassnacht, A. Walch, M. Kroiss, Endocrinology 2018.
C. Urban, A. Buck, J. T. Siveke, F. Lordick, B. Luber, A. Walch, M. Aichler, Biochim Biophys Acta 2018, 1862, 51-60.
M. Aichler, T. Kunzke, A. Buck, N. Sun, M. Ackermann, D. Jonigk, A. Gaumann, A. Walch, Lab Invest 2018, 98, 141-149.
S. Richter, …, M. Peitzsch, …, M. Kroiss, F Beuschlein, …, J Lenders, G Eisenhofer, B. Klink. Metabolome-guided genomics to identify mutations in isocitrate dehydrogenase, fumarate hydratase and succinate dehydrogenase genes in pheochromocytoma and paraganglioma, Genetics in Medicine 2018, in press.